HPLC has been used for many years and the technique is widely found in pharmacopoeia monographs. The main difference between HPLC and UPLC is the pressure that the systems run at. UPLC was created along with the development of submicron (<2 microns) LC columns to increase the efficiency of liquid chromatography separations.
Increasing the surface area of the column allows for shorter columns to be used which reduce the separation time and run times without any reduction of the resolution between compounds, often with improved resolution.
Both techniques are widely used for organic and inorganic materials. They are especially useful for determining purity, monitoring reactions and production processes and to characterise complex materials.
Our Chromatography Laboratory has a wide of range of detectors available to accommodate the needs of our clients, including:
- UV (Single wavelength)
- Photo Diode Array (PDA)
- Refractive Index (RI)
- Evaporative Light Scattering Detector (ELSD)
- Mass Spectrometry (MS)